YFMA0037
100μL
￥1100
WB， IHC， IF
Background：
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Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some pathology factors, such as hypoxia and diabetes, increased or decreased GAPDH expression in certain cell types. |
Product Information：
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Product name: Anti-GAPDH Mouse Monoclonal Antibody Isotype: IgG Form: Lyophilized Tested applications: WB, IHC-F, IHC-P, ICC/IF Dilution Ratio: WB: 1:5000-1:20000； IHC：1:50-1:100；IF: 1:100-1:200 Species reactivity: Human, Mouse, Rat, Monkey, Dog, Chicken, Hamster, Rabbit, Pig, Sheep, Insect,Yeast Host Species: Mouse Purity: Affinity purification |
Immunogen Information：
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Immunogen: A synthetic peptide of GAPDH Calculated molecular weight: 36kDa Observed molecular weight: 36kDa Swiss Prot: P04406 Gene ID : 2597 Alternative Names: G3PD; GAPD; HEL-S-162eP |
Storage：
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Services & Support：
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1、Please do not hesitate to contact us if you have any questions. 2、Please fill in the Technical Support Request Form and email the form to support@yfxbio.com within 3months of receipt of the goods, and we will response to your inquiry within 24 hours. 3、The after-sale service channel will be shut down after 3 months. 4、Producthasnot been fully validated for medical applications. For research use only. |
Application：
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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※ IF= 8.039. Yin Yuan, Hong Yao,Manzhen Zhou, et al. Identification of a Novel Potent CYP4Z1 Inhibitor Attenuating the Stemness of Breast Cancer Cells through Lead Optimization. Journal of Medicinal Chemistry, 2022 Dec 8;65(23):15749-15769.
※ IF= 3.874. Feng Zhao,Liu‐Hua Zhou, Yu‐Zheng Ge, et al. MicroRNA‐133b suppresses bladder cancer malignancy by targeting TAGLN2‐mediated cell cycle. J Cell Physiol, 14 October 2018.
※ IF= 6.832. Haiwei Ni, Hai Qin, Cheng Sun , et al. MiR-375 reduces the stemness of gastric cancer cells through triggering ferroptosis. Stem Cell Research & Therapy, Pub Date : 2021-06-05 , DOI: 10.1186/s13287-021-02394-7.
※ IF= 2.675. Feng Zhang, Haiwei Ni, Xiaoman Li, et al. LncRNA FENDRR attenuates adriamycin resistance via suppressing MDR1 expression through sponging HuR and miR-184 in chronic myelogenous leukaemia cells. FEBS Letters, Volume593, Issue15，August 2019，Pages 1993-2007.
